Biosensors based on aptamers and gold nanoparticles

时间：2023年9月11日（周一）下午15：30

地点：笃行楼316-1会议室

报告人简介：

Juewen Liu received his Bachelor’s degree from the University of Science & Technology of China in 2000 and Ph.D. from the University of Illinois at Urbana-Champaign in 2005. He joined the University of Waterloo in 2009 and is currently a professor of chemistry. He is interested in aptamers and biosensors. He received a Fred Beamish Award (2014) and a McBryde Medal (2018) from the Canadian Society for Chemistry for his contribution in bioanalytical chemistry. He is a College member of the Royal Society of Canada. He serves as a Section Editor for Biosensors & Bioelectronics, and a Contributing Editor for TrAC Trends in Analytical Chemistry. He has published over 400 papers, receiving over 38,000 citations with an H-index of 93, and is a Clarivate Highly Cited Researcher in 2022.

报告摘要：

Interfacing DNA with gold nanoparticles (AuNPs) has produced a diverse range of biosensors, imaging agents, drug delivery systems, and new materials. AuNPs are particularly attractive because of their low toxicity and unique optical, electric and catalytic properties. Using fluorescently labeled oligonucleotides as probes, we measured the kinetics of DNA adsorption, desorption as well as adsorption isotherm as a function of salt, pH, and temperature. An important application of such fundamental studies is the attachment of DNA to AuNPs. Highly efficient DNA adsorption can be achieved at low pH and freezing. In addition, we studied aptamer-based sensors using AuNPs for signal production and revealed some potential artifacts. In the second part of the talk, our recent work on selection of DNA aptamers for binding to small molecular targets will be presented.